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CD34 + progenitor cell maintenance was impaired, aligning with observed intrinsic defects and reduced colony-forming ability in SDS patients

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

Tivantinib (ARQ197) displays Cytotoxic activity that is independent of its ability to bind MET

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